Simultaneous determination of methylenetetrahydrofolate reductase C677T and factor V G1691A genotypes by mutagenically separated PCR and multiple-injection capillary electrophoresis.

C677T mutation of methylenetetrahydrofolate reductase gene determined in blood or plasma by multiple-injection capillary electrophoresis and laser-induced fluorescence detection.

We constructed an assay to detect the common C677T mutation in the methylenetetrahydrofolate reductase gene. The mutation creates a Hinfl recognition site detected by restriction cleavage of a 198-bp fragment amplified in the polymerase chain reaction (PCR). Digested samples were subjected to capillary electrophoresis with laser-induced fluorescence detection (CE-LIF), with hydroxypropylmethylc...

C677T mutation of methylenetetrahydrofolate reductase gene determined in blood or plasma multiple-injection capillary electrophoresis and induced fluorescence detection

C677T mutation of methylenetetrahydrofolate reductase gene determined in blood or plasma multiple-injection capillary electrophoresis and induced fluorescence detection

C677T and A1298C Mutations in the Methylenetetrahydrofolate Reductase Gene in Patients with Recurrent Abortion from the Iranian Azeri Turkish

Background To assess whether the C677T and A1298C mutations in the methylenetetrahydrofolate reductase (MTHER) gene are associated with recurrent abortion (RA), we determined the frequencies of the T677 and C1298 mutations in patients and controls. MaterialsAndMethods Mutations were determined by a RFLP-PCR method in 53 patients and 61 matched controls. Results The frequencies of T alleles were...

The Insulin-like Growth Factor-1 (G>A) and 5,10-methylenetetrahydrofolate Reductase (C677T) Gene Variants and the Serum Levels of Insulin-like Growth Factor-1, Insulin, and Homeostasis Model Assessment in Patients with Acne Vulgaris

Background & Objective: To find an association between gene variants of insulin-like growth factor-1 (IGF-1) and 5,10-methylenetetrahydrofolate reductase (MTHFR) with the risk of acne vulgaris (AV). Methods: In a case-control study, we investigated 150 AV patients and 148 healthy individuals (aged 18-25 years) for the IGF-1 G>A and MTHFR C677T polymorphis...